NON-HODGKIN’S LYMPHOMAS

Follicular lymphoma
t (14; 18) IGH- BCL2
Mantle cell Lymphoma
t (11; 14) IgH-CCND1
Burkitt Lymphoma
C-MYC rearrangement
Anaplastic Lymphoma
ALK rearrangement
Other Lymphoma
MALT BCL2- BCL6 rearrangement
Formalin-Fixed Paraffin-Embedded Tissue Store at room temperature 15 days
Whole Blood Collect on Heparin
Transport Immediately or Refrigerate 4ºC
Transport on ice within 24H
15 days
JAK2
Gene- Val617PHE(V17F) mutation
Whole Blood Collect on Heparin
Transport Immediately or Refrigerate at 4ºC Transport on ice within 24H
15 days
BRAIN CANCER

Glioma
1p36 + 19q Combined Deletion
Menigioma
1p36 deletion
Aneuploidy determination (14-22)
p16 deletion
Medulloblastoma :
N-MYC and C-MYC Amplification
Formalin-Fixed Paraffin-Embedded Tissue Store at room temperature 15 days
SOFT TISSUE TUMORS

Ewing Sarcoma and PNET
EWS rearrangement
Liposarcoma
MDM2 amplification
Myxoid Liposarcoma
CHOP rearrangement
FUS rearrangement
Alveolar Rhabdomyosarcoma
FKHR rearrangement
Synovialsarcoma
SYT rearrangement
Inflammatory Myofibroblastic Tumor
ALK rearrangement
Formalin-Fixed Paraffin-Embedded Tissue Store at room temperature 15 days
HEMATOPOEITIC PATHOLOGY

BCR-ABL
PML-RARA t(15 ;17)
Whole Blood or Bone Marrow
Collect on EDTA Transport Immediately or Refrigerate at 4ºC Transport on ice within 24H 15 days
GENE REARRANGEMENTS
Deletion., amplification
Formalin-Fixed Paraffin-Embedded Tissue
Store at room temperature 15 days
NEUROBLASTOMA

N-MYC amplification
1p36 deletion
Ploidy determination
Formalin-Fixed Paraffin-Embedded Tissue
Store at room temperature 15 days
PRENATAL CYTOGENETIC

Detection of aneuploidy Trisomy 13, 18, 21, X and Y
Amnoitic Fluid Collect In Sterile Tube, Refrigerate at 4ºC Transport within on Ice 48hrs 15 days
Oncotype DX Formalin-Fixed Paraffin-Embedded Tissue
Store at room temperature 15 days
Kras Mutations Formalin-Fixed Paraffin-Embedded Tissue
Store at room temperature 15 days
EGFR Formalin-Fixed Paraffin-Embedded Tissue
Store at room temperature 15 days
Chlamydia Trachomatis Endocervical Scrape/
Discharge Scrape
Store In PBS, Refrigerate at 4ºC, Transport On Ice within 48hrs. 5 days
Urine 20-30 ml Early Morning Urine In Sterile Container, Refrigerate at 4ºC, Transport On Ice within 48hrs. 5 days
EBV
Real-Time PCR
Serum 2-3ml
Plasma 2-3ml
Cerebrospinal Fluid 1-2ml
Amniotic Fluid 10-15ml
Collect In Sterile Tube, Refrigerate at 4ºC, Transport On Ice within 48hrs. 7 days
Hepatitis Virus B Quantitative
Real-Time PCR
Serum 2-3ml Collect In Sterile Tube, Refrigerate at 4ºC, Transport On Ice within 48hrs. 7 days
Hepatitis Virus C Quantitative
Real-Time PCR
Serum 2-3ml Collect In Sterile Tube, Refrigerate at 4ºC, Transport On Ice within 48hrs. 7 days
Hepatitis Virus C Genotype
Real Time PCR (Genotypes 1 - 6)
Serum 2-3ml Collect In Sterile Tube, Refrigerate at 4ºC, Transport On Ice within 48hrs. 7 days
Herpes Simplex Virus 1/2 Cervicovaginal Secretions Store In PBS, Refrigerate at 4ºC, Transport On Ice within 48hrs. 5 days
Cutaneous Lesion Specimen Should be preserved in formalin 10%. 5 days
Respiratory Samples Cerebrospinal Fluid 1-2m Collect In Sterile Tube, Refrigerate at 4ºC, Transport within 48hrs. 5 days
Human Cytomegalovirus
Real-Time PCR
Serum 2-3ml
Plasma 2-3ml
Cerebrospinal Fluid 1-2ml
Amniotic Fluid 10-15ml
Collect In Sterile Tube, Refrigerate at 4ºC, Transport on ice within 48hrs. 7 days
Human Papillomavirus
Detect +/- Type
Fresh Cervical Scrape Store In PBS, Refrigerate at 4ºC, Transport On Ice within 48hrs. 5 days
Biopsy Specimen Should be preserved in formalin 10%. 5 days
Urine 20- 30 ml Early Morning Urine In Sterile Container, Refrigerate at 4ºC, Transport On Ice within 48hrs. 5 days
Measles Virus Quantitative
Real-Time PCR
Serum 2-3ml Collect In Sterile Tube, Refrigerate at 4ºC, Transport on ice within 48hrs. 5 days
Mycobacterium Tuberculosis
Real-Time PCR
Respiratory Samples; BAL / Sputum
Fresh / FFPET Respiratory Biopsy
Cerebrospinal Fluid 1-2ml
Pleural Fluid
Collect In Sterile Tube, Refrigerate at 4ºC, Transport on ice within 48hrs. 5 days
Formalin-Fixed Paraffin-Embedded Tissue Store at room temperature 5 days
STD-Multipathogen Testing
Mycoplasma hominis
Ureaplasma urealyticum
Neisseria gnonrrhoeae
Endocervical Scrape Store In PBS, Refrigerate at 4ºC. Transport On Ice within 48hrs. 5 days
Urine 20-30 ml Collect Urine In Sterile Container, Refrigerate at 4ºC, Transport On Ice within 48hrs. 5 days
Toxoplasma Gondi
Real-Time PCR
Whole Blood
Cerebrospinal Fluid 1-2ml
Respiratory Sample
Amniotic Fluid
Collect In Sterile Tube, Refrigerate at 4ºC, Transport on ice within 48hrs. 7 days
Formalin-Fixed Paraffin-Embedded Tissue Store at room temperature 7 days
Varicella Zoster Virus
Real-Time PCR
Amniotic fluid 10-15ml
Serum 2-3ml
Respiratory Samples
Cerebrospinal Fluid 1-2ml
Collect In Sterile Tube, Refrigerate at 4ºC, Transport On Ice within 48hrs. 7 days